Publications

Conserved NT5C2 links context-specific behaviors with psychiatric and metabolic risk

Moulin TC, Aldavero-Muñoz I, Williams MJ, Schiöth HB

“Feeding behavior was quantified using the FlyPad system, a capacitive-based platform that records proboscis-food interactions [36].”

Aversive learning hijacks a brain sugar sensor to consolidate memory

Francés R, Comyn T, Desnous C, Bettoni F, Pavlowsky A, Préat T, Plaçais P

“The food-choice assay was done using FlyPAD 44 according to the manufacturer's instructions.”

Reduced dopaminergic reinforcement, not learning capacity, limits operant learning in aging Drosophila

Jahan I, Holvoet H, Backer JD, Kadow ICG

“Here, we used a closed-loop optogenetic paradigm to test how aging affects operant learning and the role of dopaminergic neurons (DANs; PPL1 and PAM) in this process.”

Genetic sex of enteric neurons enables ovarian relaxin to gate maternal gut plasticity

Silva B, Tsakani E, Kontopoulos A, De J, Gaspar P, Hiridjee A, Rosselli C, Juan-Sanz JD, Campusano J, Treiber CD, et al.

“FlyPAD assays were performed as previously described 1,17,74 .”

SkitoSnack 2.0 - A Bloodmeal Alternative for Anopheles and Aedes Mosquitoes

Karki A, Luker HA, Potlapalli NNR, Holguín FO, Berwal M, Pietrantonio PV, Hansen IA

“We used the flyPAD feeding system that was originally developed for Drosophila melanogaster and later adapted to study mosquito feeding behavior [29,30].”

Feeding decision-making by a single neuron via disparate neurotransmitters

Savaş D, Okoro AM, Moșneanu RA, Crown AM, Chang Z, Siegel R, Sorkaç A, Zandawala M, Barnea G

“We then show that SELKs affect bitter avoidance through LK release and food intake in an acetylcholine-dependent manner.”

Role of dopamine signaling in male courtship suppression induced by confinement stress in Drosophila

Sato T, Toyama R, Kitamoto T, Sakai T

“After a 23-h fasting period, feeding activity (number and duration of sips) was monitored for 1 h after 1-h SS stress.”

Tumor-induced orexigenic imbalance lowers protein appetite and drives early organ wasting symptoms

Petsakou A, Filine E, Li M, Chen Y, Zheng A, Perrimon N

“To test this, we used the FlyPAD to conduct a two-choice behavioral assay for the two main macronutrients in the fly's diet, protein and sugar (yeast vs sugar preference assay).”

The RASA2/RASA3 ortholog RasGAP1 modulates obesity-linked phenotypes and is associated with leptin-analog signaling in Drosophila

Moulin TC, Williams MJ, Schiöth HB

“The primary outcome measure was the number of sips, defined as a single proboscis-food interaction, which is shown to correlate with food intake volume.”

Quantifying Drosophila Feeding Behavior Using flyPAD and optoPAD

Collins NJ, Endres MN, Sinakevitch IT, Shao L

“Here, we describe a detailed protocol for performing consumption and dietary choice assays in Drosophila using the flyPAD/optoPAD system.”

Mating-dependent lifespan cost of sterol depletion in male Drosophila melanogaster

McCracken AW, White N, Tanianis-Hughes J, Rosca E, Gill T, McKinney L, Simpson SJ, Reinhardt K, Brankatschk M, Piper MDW, et al.

“We first measured dietary consumption using a Flypad automatic sip detection system (31).”

Protein-responsive gut hormone tachykinin directs food choice and impacts lifespan

Ahrentløv N, Kubrak O, Lassen M, Malita A, Koyama T, Frederiksen AS, Sigvardsen CM, John A, Madsen PSMSM, Halberg KA, et al.

“1-h feeding behaviour (‘sips’) was recorded using the flyPAD apparatus 26 .”

Adenosine signaling in glia modulates metabolic state-dependent behavior in Drosophila

Backer JD, Karges T, Papst J, Pınar ZN, Coman C, Ahrends R, Xu Y, García‐Cáceres C, Kadow ICG

“In food-deprived flies, astrocyte-like and perineurial glia promote foraging and feeding, respectively, while cortex glia suppress these behaviors.”

Evolution of taste processing shifts dietary preference

Bertolini E, Münch D, Pascual J, Sgammeglia N, Bruzzone M, Ribeiro C, Auer TO

“We next analysed taste-driven noni preference in three binary choice assays, a 72-well plate assay, a petri dish group assay and the fly-PAD (on single flies), comparing grape and noni juice.”

Connectomics Reveals a Feed-Forward Swallowing Circuit Driving Protein Appetite

Tastekin I, Vicente IDH, Beresford RJ, Otto N, Dempsey G, Waddell S, Ribeiro C

“flyPAD assays: Feeding microstructure was monitored using flyPAD as described in Itskov et al. Female flies ... were placed in an arena with two electrode pairs (channels) filled with food patches.”

Dual roles of Drosophila reward-encoding dopamine neurons in regulating innate and learned behaviors

Lozada-Perdomo FV, Chen Y, Jacobs RV, Yeo J, Yang M, Bhalerao J, Devineni AV

“Detailed information regarding the design of the optoPAD and data processing methods can be found in Moreira et al. and Itskov et al. Briefly, each fly was placed in a 3 cm diameter circular arena with two feeding sensors on the floor.”

La Jolla Virus: The Pathology and Transmission in Its Host Drosophila suzukii

Abdelhafiz I, Kessel T, Vilcinskas A, Lee K

“To assess the impact of viral infection on feeding behavior, we employed the flyPAD (fly Proboscis and Activity Detector) system, an automated high-resolution behavioral monitoring tool... in Drosophila [24].”

Dual roles of Drosophila reward-encoding dopamine neurons in regulating innate and learned behaviors

Lozada-Perdomo FV, Chen Y, Jacobs RV, Yeo J, Yang M, Bhalerao J, Devineni AV

“activating the entire population of reward-encoding DANs in Drosophila drives innate aversion in multiple behavioral assays, including feeding, locomotion, and spatial preference”

From perception to valence: a pair of interneurons that assign positive valence to sweet sensation in Drosophila

Christie KW, Dadyala TS, Sinakevitch I, Ito M, Shao L

“Acute feeding behavior was quantified by use of the FlyPad behavioral monitoring system (Itskov et al. 2014), at 25°C, 50%-60% relative humidity.”

A high-protein diet-responsive gut hormone regulates behavioral and metabolic optimization in Drosophila melanogaster

Yoshinari Y, Nishimura T, Yoshii T, Kondo S, Tanimoto H, Kobayashi T, Matsuyama M, Niwa R

“FlyPAD assays were performed according to a previously described method.”

Mating reconciles fitness and fecundity by switching diet preference in flies

Liu CF, Tian N, Chang PW, Zhang W

“The sips' signal was analyzed using MATLAB and calculated as described in a previous study 55.”

The caloric value of food intake structurally adjusts a neuronal mushroom body circuit mediating olfactory learning inDrosophila

Çoban B, Poppinga H, Rachad EY, Geurten BRH, Vasmer D, Jimenez FJR, Gadgil YV, Deimel SH, Alyagor I, Schuldiner O, et al.

“Five microliters of 1 M sucrose...were placed in wells of the FlyPad...to detect the capacitance change when the flies physically interacted with the food. The flies were transferred to the FlyPad individually using a pump.”

Protein-responsive gut hormone Tachykinin directs food choice and impacts lifespan in Drosophila

Rewitz K, Ahrentløv N, Kubrak O, Malita A, Koyama T, John A, Madsen PSMSM, Halberg KA, Nagy S, Texada MJ

“The flyPAD apparatus was also used for sugar and yeast feeding in a two-choice behavioral assay... was loaded into the food pedestals of each flyPAD. Flies were...transferred into the flyPAD behavioral arenas.”

Genetic Variation in Trophic Avoidance Behaviour Shows Fruit Flies are Generally Attracted to Bacterial Substrates

Monteith KM, Thornhill P, Vale PF

“Using the FlyPAD system, we tracked the feeding choice that flies made between substrates that were either clean or contained a bacterial pathogen.”

Taste cells expressing Ionotropic Receptor 94e reciprocally impact feeding and egg laying in Drosophila

Guillemin J, Li V, Davis G, Audette K, Li J, Jelen M, McDowell SA, Slamani S, Kelliher L, Gordon MD, et al.

“This was achieved by using either the opto-lid FlyPad system (STROBE) 34,37 or the opto-lid FLIC system 35,36 over two hours.”

Anti-diuretic hormone ITP signals via a guanylate cyclase receptor to modulate systemic homeostasis in Drosophila

Gera J, Agard M, Nave H, Baldridge AB, Sajadi F, Thorat L, McKim TH, Kondo S, Nässel DR, Omar MH, et al.

“flyPAD (Itskov et al., 2014) was used to calculate the number of feeding bouts over 24 hours as well as preference between sucrose versus yeast. Individual flies were mouth-pipetted to a flyPAD unit.”

Anti-diuretic hormone ITP signals via a guanylate cyclase receptor to modulate systemic homeostasis in Drosophila

Gera J, Agard M, Nave H, Baldridge AB, Sajadi F, Thorat L, McKim TH, Kondo S, Nässel DR, Omar MH, et al.

“flyPAD (Itskov et al., 2014) was used to calculate the number of feeding bouts over 24 hr as well as preference between sucrose versus yeast. Individual flies were mouth-pipetted to a flyPAD unit.”

Molecular characterization of gustatory second-order neurons reveals integrative mechanisms of gustatory and metabolic information

Mollá-Albaladejo R, Jiménez-Caballero M, Sánchez‐Alcañiz JA

“flyPAD assays were performed to study the feeding microstructure in a two-choice feeding paradigm as described previously (Itskov et al., 2014), and several feeding parameters were measured individually in a high-throughput manner.”

Genetic variation in trophic avoidance shows fruit flies are generally attracted to bacterial pathogens

Monteith KM, Thornhill P, Vale PF

“We designed a two-choice feeding assay using the FlyPAD system for tracking, recording, and analysing the feeding behaviour of fruit flies in real-time [42].”

Adenosine signaling in glia modulates metabolic state-dependent behavior in Drosophila

Backer JD, Karges T, Papst J, Coman C, Ahrends R, Xu Y, García‐Cáceres C, Kadow ICG

“We optogenetically activated glial cells on the FlyPAD when the fly's proboscis contacted the drop of sucrose (Figure 5C).”

Lack of Single Amino Acids Transcriptionally Remodels Sensory Systems to Enhance the Intake of Protein and Microbiota

Ezra-Nevo G, Henriques SF, Münch D, Francisco AP, Baltazar C, Elias AP, Deplancke B, Ribeiro C

“Food choice experiments were done using flyPAD as described in 118.”

A high-protein diet-responsive gut hormone regulates behavioural and metabolic optimization in Drosophila melanogaster

Niwa R, Yoshinari Y, Nishimura T, Yoshii T, Kondo S, Tanimoto H, Kobayashi T, Matsuyama M

“FlyPAD assays were performed according to previously described method. A single fly starved in 1% agar for 16 h was placed in an arena with either 5% sucrose or 2.5% yeast autolysate solution.”

Confinement stress with movement restriction suppresses male courtship in Drosophila through dopamine-dependent neuroplasticity

Sato T, Toyama R, Kitamoto T, Sakai T

“Using an automatic feeding monitoring system, FlyPAD, we measured the number of sips and sip duration in individual males... the 1 h feeding behavior of males was monitored using FlyPAD.”

A neuronal substrate for translating nutrient state and resource density estimations into foraging decisions

Goldschmidt D, Tastekin I, Münch D, Park J, Haberkern H, Serra L, Baltazar C, Jayaraman V, Rubin GM, Ribeiro C

“The absence of a change in overall food intake was further corroborated by using the flyPAD assay (Itskov et al., 2014), which directly measures the total amount of feeding.”

Selective integration of diverse taste inputs within a single taste modality

Deere JU, Sarkissian AA, Yang M, Uttley H, Santana NM, Nguyen L, Ravi K, Devineni AV

“To examine more naturalistic feeding behavior in freely moving flies over a longer time period, we used the optoPAD assay (Moreira et al., 2019). The optoPAD was purchased from Pavel Itskov at Easy Behavior.”

Optimized design and in vivo application of optogenetically functionalized Drosophila dopamine receptors

Zhou F, Tichy A, Imambocus BN, Sakharwade S, Jiménez F, Martínez MG, Jahan I, Habib M, Wilhelmy N, Burre V, et al.

“5 ul of 10% sucrose ... were placed in wells of the flyPAD ... For the optoPAD experiments, flies were ... supplemented with all-trans-retinal ... a closed-loop system to stimulate flies during feeding.”

Automated analysis of feeding behaviors of females of the mosquito Aedes aegypti using a modified flyPAD system

Henriques‐Santos BM, Xiong C, Pietrantonio PV

“For the behavioral experiments, the flyPAD automated monitoring system of feeding behavior developed for adult Drosophila flies 43 was adapted for female mosquitoes (Fig.”

Serotonergic control of feeding microstructure in Drosophila

Banu A, Gowda SBM, Salim S, Mohammad F

“We used the FlyPAD, a capacitance-based system, to measure fly-feeding behavior in a detailed, high-throughput manner (Itskov et al., 2014).”

A dedicate sensorimotor circuit enables fine texture discrimination by active touch

Yu J, Guo X, Shen Z, Zhang W

“We then validated these findings by using an automatic feeding monitoring system (FlyPAD) [26] to quantify the total duration of the fly sip within 20 minutes.”

Optimized design and in vivo application of optogenetically functionalized Drosophila dopamine receptors

Zhou F, Tichy A, Imambocus BN, Jiménez F, Martínez MG, Jahan I, Habib M, Wilhelmy N, Bräuler V, Lömker T, et al.

“We chose an optoPAD setup which allows operant optogenetic stimulation of flies during feeding using a closed-loop system.”

A gut-derived hormone suppresses sugar appetite and regulates food choice in Drosophila

Malita A, Kubrak O, Koyama T, Ahrentløv N, Texada MJ, Nagy S, Halberg KA, Rewitz K

“we used a second automated behaviour-monitoring apparatus, the flyPAD 28 , to measure feeding preference in a two-choice assay.”

Bacteria-derived peptidoglycan triggers a non-canonical NF-κB dependent response in Drosophila gustatory neurons

Masuzzo A, Manière G, Grosjean Y, Kurz CL, Royet J

“Two-choice feeding assays were performed by using the flyPAD device (Itskov et al., 2014), which records the cumulative number of sips.”

Disproportionate investment in Spiralin B production limits in-host growth and favors the vertical transmission of Spiroplasma insect endosymbionts

Masson F, Rommelaere S, Schüpfer F, Boquete J, Lemaître B

“We assessed the impact of MSRO infection on fly feeding behavior with a FlyPad (18).”

Bacterial Metabolism and Transport Genes Are Associated with the Preference of Drosophila melanogaster for Dietary Yeast

Call TB, Davis EK, Bean JD, Lemmon SG, Chaston JM

“We measured dietary preference of individual 4- to 6-day-old female flies using flyPAD arenas (6).”

marmite defines a novel conserved neuropeptide family mediating nutritional homeostasis

Francisco AP, Tastekin I, Fernandes AB, Ezra-Nevo G, Deplancke B, Oliveira‐Maia AJ, Gontijo AM, Ribeiro C

“we decided to test for phenotypes in feeding behavior and food choice using the flyPAD technology, a capacitance-based setup for testing independent changes in protein and carbohydrate feeding (Itskov et al., 2014).”

Selective integration of diverse taste inputs within a single taste modality

Deere JU, Sarkissian AA, Yang M, Uttley H, Santana NM, Nguyen L, Ravi K, Devineni AV

“To examine more naturalistic feeding behavior in freely moving flies over a longer time period, we used the optoPAD assay (Moreira et al., 2019).”

A neural circuit linking two sugar sensors regulates satiety-dependent fructose drive in Drosophila

Musso P, Junca P, Gordon MD

“Together, our results demonstrate how a three-layer neural circuit links the detection of two sugars to produce precise satiety-dependent control of feeding behavior.”

A glucose-supplemented diet enhances gut barrier integrity in Drosophila

Galenza A, Foley E

“To measure feeding frequency, we used the flyPAD (Itskov et al., 2014) to count individual sips; bursts, which are clusters of sips; and bouts, which are clusters of bursts.”

Distinct internal states interact to shape food choice by modulating sensorimotor processing at global and local scales

Münch D, Goldschmidt D, Ribeiro C

“we used thermogenetic activation of the labeled neurons in combination with the flyPAD feeding assay (Itskov et al., 2014).”

Multidrug Resistance Like Protein 1 Activity in Malpighian Tubules Regulates Lipid Homeostasis in Drosophila

Liu W, Cao H, Kimari M, Maronitis G, Williams MJ, Schiöth HB

“We performed FlyPAD experiments using starved flies... To do this, we employed the Fly Proboscis and Activity Detector (flyPAD). The flyPAD enables real-time recording of food intake and feeding behavior with a high temporal resolution.”

Modulation of taste sensitivity by the olfactory system in Drosophila

Junca P, Stanley M, Musso P, Gordon MD

“FlyPAD assays were performed similarly to those previously described. Flies were individually transferred to flyPAD arenas by mouth aspiration and allowed to feed for one hour at 25C, 70% RH. FlyPAD data were acquired using the Bonsai framework.”

A neural circuit linking two sugar sensors regulates satiety-dependent fructose drive in Drosophila

Musso P, Junca P, Gordon MD

“we silenced them and measured feeding using a modified version of FlyPAD where food interactions were calculated using the algorithm we developed for the STROBE (Figure 2B-C) (Itskov et al., 2014; Jaeger et al., 2018; Musso et al., 2019).”

Bacteria-derived peptidoglycan triggers an NF-κB dependent response in Drosophila gustatory neurons

Masuzzo A, Manière G, Grosjean Y, Kurz CL, Royet J

“Two-choice feeding assays were performed by using the flyPAD device which records the cumulative number of sips. Each sip corresponds to a contact of flies' proboscis with the chosen food substrate.”

Metabolic cross-feeding in imbalanced diets allows gut microbes to improve reproduction and alter host behaviour

Henriques SF, Dhakan DB, Serra L, Francisco AP, Carvalho-Santos Z, Baltazar C, Elias AP, Anjos M, Zhang T, Maddocks OD, et al.

“Lactate is also necessary and sufficient for Ap to alter the fly's protein appetite.”

Enteric neurons increase maternal food intake during reproduction

Hadjieconomou D, King GA, Gaspar P, Mineo A, Blackie L, Ameku T, Studd C, Mendoza AD, Diao F, White BH, et al.

“Food intake and feeding behaviour assays - FlyPAD: FlyPAD assays were performed as described in ref. 37. ... flies were individually transferred to flyPAD arenas by mouth aspiration and allowed to feed for 1h.”

Visceral Mechano-sensing Neurons Control Drosophila Feeding by Using Piezo as a Sensor

Wang P, Jia Y, Liu T, Jan YN, Zhang W

“To avoid the influence on feeding by manual handling, we used an automatic feeding monitoring system (FlyPAD) (Itskov et al., 2014) to quantify the total sip duration of the flies within 20 min.”

Immune Receptor Signaling and the Mushroom Body Mediate Post-ingestion Pathogen Avoidance

Kobler JM, Jiménez F, Petcu I, Kadow ICG

“we tested different feeding choices involving harmless and pathogenic bacterial strains in two-choice feeding assays: a slightly modified version of the CAFE (STAR Methods) and the flyPAD.”

CCAP regulates feeding behavior via the NPF pathway in Drosophila adults

Williams MJ, Akram M, Barkauskaite D, Patil S, Kotsidou E, Kheder S, Vitale G, Filaferro M, Blemings SW, Maestri G, et al.

“The feeding experiments for preference testing were performed using the flyPAD (35).”

Transient Administration of Dopaminergic Precursor Causes Inheritable Overfeeding Behavior in Young Drosophila melanogaster Adults

Moulin TC, Ferro F, Berkins S, Hoyer A, Williams MJ, Schiöth HB

“To assess feeding behavior, we used the FlyPad, a recently-developed equipment based on the proboscis interaction with the food [23].”

Endocrine fine-tuning of daily locomotor activity patterns under non-starving conditions in Drosophila

Pauls D, Selcho M, Räderscheidt J, Amatobi KM, Fekete G, Krischke M, Hermann-Luibl C, Ünal AG, Ehmann N, Itskov PM, et al.

“To directly address food uptake, we used an automated two-choice flyPAD assay and monitored feeding in individual flies on 5mM sucrose.”

Long-lasting impact of starvation experience on fly activity and place preference

Mahishi D, Triphan T, Hesse R, Huetteroth W

“flyPAD Food sip measurements were performed on the flyPAD (Itskov et al., 2014).”

Sex Differences in Intestinal Carbohydrate Metabolism Promote Food Intake and Sperm Maturation

Hudry B, Goeij ED, Mineo A, Gaspar P, Hadjieconomou D, Studd C, Mokochinski JB, Kramer H, Plaçais P, Préat T, et al.

“Using flyPAD to monitor feeding behavior in freely behaving flies (Itskov et al., 2014), we observed that reduced JAK-STAT signaling in male ECs resulted in reduced food intake.”

Immune Regulation of Intestinal Stem Cell Proliferation and Differentiation in Drosophila

Shin M, Ferguson M, Willms RJ, Jones LO, Petkau K, Foley E

“Drosophila feeding behavior was monitored for 1 hour on the FlyPad as described previously (66).”

Metabolic cross-feeding allows a gut microbial community to overcome detrimental diets and alter host behaviour

Henriques SF, Serra L, Francisco AP, Carvalho-Santos Z, Baltazar C, Elias AP, Anjos M, Zhang T, Maddocks OD, Ribeiro C

“Food choice experiments were performed using the flyPAD as described in (Itskov et al., 2014).”

Cellular metabolic reprogramming controls sugar appetite

Carvalho-Santos Z, Cardoso-Figueiredo R, Elias AP, Ribeiro C

“We tested these females for sugar appetite phenotypes using the flyPAD technology (Itskov et al., 2014).”

An expression atlas of variant ionotropic glutamate receptors identifies a molecular basis of carbonation sensing

Sánchez‐Alcañiz JA, Silbering AF, Croset V, Zappia G, Sivasubramaniam AK, Abuin L, Sahai SY, Münch D, Steck K, Auer TO, et al.

“flyPAD assays were performed using only one well of the arena per assay.”

Internal amino acid state modulates yeast taste neurons to support protein homeostasis in Drosophila

Steck K, Walker SJ, Itskov PM, Baltazar C, Moreira JM, Ribeiro C

optoPAD: a closed-loop optogenetics system to study the circuit basis of feeding behaviors

Moreira J, Itskov PM, Goldschmidt D, Steck K, Walker SJ, Ribeiro C

“optoPAD assays were performed following a protocol previously described (Itskov et al., 2014).”

The STROBE: a system for closed-looped optogenetic control of freely feeding flies

Musso P, Junca P, Jelen M, Feldman-Kiss D, Zhang H, Chan RC, Gordon MD

“The hardware, with the exception of the lighting circuit units, is based on the FlyPAD design (Itskov et al., 2014).”

Commensal bacteria and essential amino acids control food choice behavior and reproduction

Leitão-Gonçalves R, Carvalho-Santos Z, Francisco AP, Fioreze GT, Anjos M, Baltazar C, Elias AP, Itskov PM, Piper MDW, Ribeiro C

“We show that in Drosophila melanogaster, essential amino acids (eAAs) and the concerted action of the commensal bacteria Acetobacter pomorum and Lactobacilli are critical modulators of food choice.”

Matching Dietary Amino Acid Balance to the In Silico-Translated Exome Optimizes Growth and Reproduction without Cost to Lifespan

Piper MDW, Soultoukis GA, Blanc E, Mesaros A, Herbert SL, Juricic P, He X, Atanassov I, Salmonowicz H, Yang M, et al.

“Mated adult flies were then maintained on either holidic or yeast-based medium for the pretreatment period (3 days) and assayed using flyPAD, as described in Itskov et al. (2014).”

Gonadal ecdysone titers are modulated by protein availability but do not impact protein appetite

Carvalho-Santos Z, Ribeiro C

“we also tested these flies for alterations in food choice using the flyPAD technology, which allows for the automatic and quantitative survey of the feeding interactions of single flies with two different nutrient sources (Itskov et al., 2014).”

Internal amino acid state modulates yeast taste neurons to support protein homeostasis in Drosophila

Steck K, Walker SJ, Itskov PM, Baltazar C, Ribeiro C

“flyPAD assays were performed based on a protocol previously described. One well of the flyPAD was filled with 20 mM sucrose, and the other with 10% yeast... Flies were individually transferred to flyPAD arenas by mouth aspiration.”

Internal states drive nutrient homeostasis by modulating exploration-exploitation trade-off

Corrales-Carvajal VM, Faisal AA, Ribeiro C

“To precisely measure the feeding behavior of flies with different internal states, we used the flyPAD technology, which allowed us to decompose the feeding motor pattern into "sips".”